The best Side of attune flow cytometer
The best Side of attune flow cytometer
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Details-enriched characterization of cell populations. Morphological information from photos can increase to your richness of flow cytometry info. By way of example, the determine reveals an or else common apoptosis assay working with Annexin V and PI, adding mobile imaging to characterize cells in Each and every inhabitants to reveal morphologically unique capabilities. These insights could not are already obtained from multiplex staining alone.
As samples are obtained about the Attune CytPix Flow Cytometer, the superior-velocity brightfield digital camera captures and suppliers visuals of detected situations at a charge up to six,000 photos per next, dependant upon the flow price and picture size. For larger adaptability, Attune Cytometric Application lets you regulate the impression seize frequency as needed and select a specific gate for picture seize, if needed.
(B) Uncooked 264.7 macrophages are sometimes annotated inaccurately with regard to particle rely because of their complexity and noncircular borders. The number of singlets available for analysis increases after the consumer trains and employs a more accurate design.
Learn new insights into your experimental details by visualizing populations working with combined fluorescence and morphology info. Our software package interface wizards enable downsampling, concatenation (merged data files) and dimensionality tools which have been constructed in to the software program for an intuitive, streamlined information Investigation knowledge.
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In this article, a highly skilled person has gated singlets confidently. Soon after evaluating the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate incorporates a lot more than four% aggregates.
Pair your Attune flow cytometer having an autosampler for improved workflow efficiency, and insert robotic integration for optimum throughput.
Reliable impression good quality even at superior throughput Acoustic concentrating plus a superior-speed digicam Blend to picture these T cells regularly at lower or large flow charges. Effortlessly alter emphasis and digital camera configurations to fulfill experimental necessities.
The Attune NxT cytometer is definitely the dependable workhorse instrument your lab can depend on for dependable and trustworthy efficiency. The Attune CytPix design features a large-pace brightfield digicam and AI-pushed graphic Assessment software package, with modern updates which includes machine Studying applications and an intuitive person interface, enabling Improved details precision, accuracy, and quicker insights into sample biology.
Perhaps most significantly, these functions contain cells of Evidently unique phenotypes which may lead to incorrect conclusions concerning double constructive events (specifically in scarce populations).
Sound waves help specific cell alignment, resulting in exceptional information good quality and ~10X a lot quicker sample flow rates.
We Beforehand demonstrated the power of imaging Auto-T/Ramos cell interactions. Permit’s look at just the inhabitants of biggest fascination, the double constructive situations, to learn more. We can easily now use extended morphological parameters (listed here, circularity and skewness of depth) to even further take a look at the capabilities of these populations and refine gating on these gatherings, raising information robustness.
Designed-in dimensionality reduction equipment enhanced resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to verify predicted population resolution in lysed whole human blood. User can watch situations from Every single cluster for Visible confirmation of cell phenotypes.
Versatile payment strategy from Agilent Financial Remedies permit you to have the essential instrumentation you may need for quick project, or to increase your lab.
At the center of every Attune Flow Cytometer lies our innovative acoustic concentrating know-how, which makes use of sound waves to meticulously align cells within the flow cell. This impressive approach surpasses regular hydrodynamic procedures, resulting in speedier processing speeds, Outstanding data high-quality, and increased sample check here throughput.
Because the mobile line experienced Earlier been analyzed around the Attune CytPix Flow Cytometer, the scientists went back to the images and have been in the position to document the microbial an infection at the very least five times earlier.
Agilent Price Promise assures you at the least 10 years of instrument use through the date you buy. If not, We'll credit you While using the residual price of the process towards an upgraded design.
What appeared to be a homogeneous populace is in fact more numerous—an insight that ought to be regarded when interpreting outcomes.